Manual QLD calculator

Maximum Likelihood Estimation (MLE) used with limiting dilutions to estimate the number of organisms in a given volume.
This implementation is written by Amogh Jalihal, https://github.com/springerlab/QuantitativeLimitingDilutions.
All calculations are made locally in web.

Experiment Shape

Enter the dilution and replicate counts once, then click or drag across cells to mark growth. Shift-drag or right-drag clears cells back to negative.

Growth grid

Positive wells are shaded, negative wells are white. Click or drag to paint positive wells. Right-click or hold Shift while dragging to paint negative wells.
Build the grid to start entering wells.

Estimate

Selected wells

None yet.

MLE N/A
95% CI N/A
Variance N/A
Summary export

Relative fit function

Updates with the selected wells
Create a grid to see the fitted upstream QLD objective.

The value "MLE" is therefore the number of organisms pipetted into the first well, and diluted with this dilution factor across a series.

"# of dilutions" is the amount of grown wells in your sample, and "# of replicates" is the amount of replicate dilution series you used.

The optimal experiment is 4 replicate dilution series, where you have done 3 experimental replicates, or in other words, you have diluted 1 treatment 12 times.